Determination Of Vitamin C In Orange Juice Lab Report

Determination Of Vitamin C In Orange Juice Lab Report

Determination of Vitamin C by redox titration

Abstract

Vitamin C (ascorbic acid), is an important nutrient essential for normal growth and

development. A powerful antioxidant which slows down damage to cells in a human's body

and plays an important role in healing infections and a creator of collagen Braun P (2001).

In this experiment, a solution containing ascorbic acid was titrated with an iodine solution to

determine the concentration of ascorbic acid in various samples of (i) vitamin C tablet, (ii) (ii)

orange juice; (iii) apple juice; and (iv) fresh fruit juice (carrot-sieved). The concentration of

ascorbic acid calculated was then compared to the reported values. The values obtained

were lower than the established values, which, may have been due to errors during the

experiment.

Introduction

Vitamin C, also known as ascorbic acid is an essential micronutrient essential for normal

metabolic functioning of the body. (Jaffe 1984). Vitamin C is generally obtained from our

food, without this, humans develop scurvy, a disease characterised by bleeding gums and

the loss of teeth, slow wound healing and subcutaneous haemorrhages. Scurvy was a

recurrent and incurable problem on lengthy sea voyages until late in the eighteenth century,

until Scottish physician, James Lind, discovered that if the sailors ate citrus fruits and fresh

vegetables the disease could be prevented. As a standard provision limes were made readily

available by the British Admiralty for all voyages "and British sailors have been called

"limeys" ever since. When the active ingredient in limes was isolated, it was named ascorbic

acid ("without scurvy") acid". (Sadava 2016).

For adult non-smoking men and women, the current recommended dietary allowance for

vitamin C to prevent the deficiency disease scurvy is shown in Table 1 below (Institute of

Medicine. Food and Nutrition Board. 2000). Vitamin C a water-soluble vitamin, is defecated

by the body through urine and sweat and consequently needs to be replaced each day Braun

P (2001).

* Adequate Intake (AI)

Vitamin C - C ₆H ₈O

₆

molar mass:

176.12g/mol

melting

point: 190 °C

Boiling point: 553 °C

Density: 1.65 g/cm³

1

Table 1: Recommended Dietary Allowances (RDAs) for Vitamin C

Age Male Female Pregnancy Lactation

0–6 months 40 mg* 40 mg*

7–12 months 50 mg* 50 mg*

1–3 years 15 mg 15 mg

4–8 years 25 mg 25 mg

9–13 years 45 mg 45 mg

14–18 years 75 mg 65 mg 80 mg 115 mg

19+ years 90 mg 75 mg 85 mg 120 mg

Smokers Individuals who smoke require 35 mg/day

more vitamin C than non-smokers

Determination Of Vitamin C In Orange Juice Lab Report

Source: https://www.studocu.com/en-gb/document/canterbury-christ-church-university/introduction-to-human-biology/turnitin-final-determination-of-vitamin-c-by-redox-titration/5645805

Share: